Aller au contenu principal
Il y a présentement des items dans votre panier d'achat.
Image de profil de Marc-André Sirard
Parce que j'aime
savoir comment ça marche
Membre de l'Acfas

Marc-André Sirard

Chercheur·euse | Université Laval

Le Dr Sirard est professeur titulaire depuis 1995, année où il a fondé le Centre de recherche en biologie de la reproduction (CRBR), qui se veut aujourd’hui le plus important centre de recherche œuvrant en ce domaine au Canada.  Il est détenteur d’une Chaire senior du Canada en génomique de la reproduction et il  dirige depuis 5 ans le réseau stratégique canadien (CRSNG) EmbryoGENE dont le mandat consiste à décrire et comprendre l’effet des techniques de reproduction assistée sur la qualité de l’embryon. Le Dr Sirard compte à son actif  plus de 232 articles scientifiques publiés dans des revues avec comités de pairs.  Son index H (53) le place en tête des scientifiques canadiens en reproduction animale et humaine. Il a réussi cet exploit grâce aux 90 étudiants gradués et stagiaires postdoctoraux qu’il a formés et qui sont les principaux auteurs de ces articles. Il a signé 12 chapitres de livres et présenté plus de 72 conférences sur invitation dans des congrès internationaux. 



Mes intérêts de recherche

la reproduction l'ovule et sa capacité à reprogrammer une cellule adulte le lien entre le follicule ovarien et la qualité de l'ovule l'épigénétique ou comment on reçoit plus que nos gènes la génomique ovarienne

Formation

  • Doctorat en Médecine Vétérinaire ( U de Montréal,1981)
  • Doctorat en Médecine expérimentale ( U Laval, 1986)

Mes contributions « Science et société »

  • Marc-André SIRARD.  «  Le 47e chromosome, évolution ou eugénisme ? «  Présentation club thématique Centre de Recherche du CHUL . Novembre 2002.

    Marc-André SIRARD«La transgénèse animale : aspects techniques, appliqués et éthiques», Congrès annuel de l’Ordre des médecins vétérinaires du Québec, St-Hyacinthe, Canada, 18 octobre 2002

    Marc-André SIRARDet Lyne Létourneau. « Le risque et le génie Génétique ». Revue Horizons 5(3) 2002. 8-10

    Marc-André SIRARD, François Pothieret  Anne Reith. «  Introduction à la transgenèse animale » dans Georges A. LEGAULT (dir), Le défi transgénique : une démarche réflexive, Sainte-Foy, Presses de l’Université Laval, 2001, pp.133-138

    Marc-André SIRARD, co-direction de l’ouvrage.  (Georges A. LEGAULT (dir), et Thérèse Leroux co-directrice) Le défi transgénique : une démarche réflexive, Sainte-Foy, Presses de l’Université Laval, 2001,

    François Pothier etMarc-André SIRARD. Des animaux modifiés sur mesure. Le Médecin Vétérinaire du Québec. 2001. 31 : 166-169.

    Marc-André SIRARD. « Les nouveaux alchimistes ». Documentaire de Guy Parent pour ACPAV et Télé Québec. Distribution Cinema Libre.

    Marc-André SIRARD« La réparation de la machine humaine ». . Conférence invité dans le cadre de la conférence annuelle de la Société Canadienne de bioéthique. Québec, Octobre 2000

    Marc-André SIRARDColloque «Animaux transgéniques et xénogreffes», 66e Congrès annuel de l’Association canadienne-française pour l’avancement des sciences (Acfas), Québec, Canada, 12 mai 1998.

    

Mes publications

publication 2010-2014

240:Dias FC, Khan MI, Adams GP, Sirard MA, Singh J. Granulosa cell function and oocyte competence: Super-follicles, super-moms and super-stimulation in cattle. Anim Reprod Sci. 2014 Jul 24. pii: S0378-4320(14)00232-2. [Epub ahead of print] PubMed PMID: 25107564

239: Macaulay AD, Gilbert I, Caballero J, Barreto R, Fournier E, Tossou P, Sirard MA, Clarke HJ, Khandjian EW, Richard FJ, Hyttel P, Robert C. The Gametic Synapse; RNA Transfer to the Bovine Oocyte. Biol Reprod. 2014 Aug 20. [Epub ahead of print] PubMed PMID: 25143353.

238)Dobbs KB, Gagné D, Fournier E, Dufort I, Robert C, Block J,SIRARD MA, Bonilla L, Ealy AD, Loureiro B, Hansen PJ. Sexual Dimorphism in Developmental Programming of the Bovine Preimplantation Embryo Caused by Colony Stimulating Factor 2. Biol Reprod.2014Jul 30..

237)SIRARD MAToward building the cow folliculome. Anim Reprod Sci. 2014 Jun 30.

236)Golini VE, Stradaioli G,SIRARD MA.Transcriptome analysis of bovine granulosa cells of preovulatory follicles harvested 30, 60, 90, and 120 days postpartum.  Theriogenology.2014Jun 4.

235)Douville G,SIRARD MA. Changes in granulosa cells gene expression associated with growth, plateau and atretic phases in medium bovine follicles. J Ovarian Res.2014May 7;7:50.

234)Shojaei Saadi HA, O Doherty AM, Gagné D, Fournier E, Grant JR,SIRARD MA,  Robert C. An integrated platform for bovine DNA methylome analysis suitable for small samples. BMC Genomics.2014Jun 9;15(1):451.

233)Labrecque R, Vigneault C, Blondin P,SIRARD MA..Gene expression analysis of bovine oocytes at optimal coasting time combined with GnRH antagonist during the no-FSH period. Theriogenology. 2014 May;81(8):1092-1002014Jan 31.

232)Pagé-Larivière F,SIRARD MA.Spatiotemporal expression of DNA demethylation enzymes and histone demethylases in bovine embryos. Cell Reprogram.2014Feb;16(1):40-53.

231)Cagnone G,SIRARD MA. The impact of exposure to serum lipids during in vitro culture on the transcriptome of bovine blastocysts. Theriogenology.2014 Mar 15;81(5):712-22

230)Van Hoeck V, Rizos D, Gutierrez-Adan A, Pintelon I, Jorssen E, Dufort I,  SIRARD MA, Verlaet A, Hermans N, Bols PE, Leroy JL. Interaction between differential gene expression profile and phenotype in bovine blastocysts originating from oocytes exposed to elevated non-esterified fatty acid concentrations. Reprod Fertil Dev.2013Dec 23.

229)Orozco-Lucero E, Dufort I, Robert C,SIRARD MA. Rapidly cleaving bovine two-cell embryos have better developmental potential and distinctive mRNA pattern. Mol Reprod Dev. 2013 Nov 7.

228)Labrecque R,SIRARD MA. The study of mammalian oocyte competence by transcriptome analysis: progress and challenges. Mol Hum Reprod.2013Nov 27.

227)Gohin M, Fournier E, Dufort I,SIRARD MA. Discovery, identification and sequence analysis of RNAs selected for very short or long poly A tail in immature bovine oocytes. Mol Hum Reprod.2013Dec 2.

226)Assidi M, Richard FJ,SIRARD MA. FSH in vitro versus LH in vivo: similar genomic effects on the cumulus. J Ovarian Res. 2013Sep 25;6(1):68.

225)Bunel A, Nivet AL, Blondin P, Vigneault C, Richard FJ,SIRARD MA. Cumulus cell gene expression associated with pre-ovulatory acquisition of developmental competence in bovine oocytes. Reprod Fertil Dev.2013Jul 5. [Epub ahead of print]

224)de Montera B, Fournier E, Shojaei Saadi HA, Gagné D, Laflamme I, Blondin P, SIRARD MA, Robert C. Combined methylation mapping of 5mC and 5hmC during early embryonic stages in bovine. BMC Genomics.2013Jun 18;14:406. [Epub ahead of print]

223)Dias FC, Khan MI,SIRARD MA, Adams GP, Singh J. Differential gene expression of granulosa cells after ovarian superstimulation in beef cattle. Reproduction.  2013Jun 6. [Epub ahead of print]

222)Nivet AL, Vigneault C, Blondin P,SIRARD MA. Changes in granulosa cells gene expression associated with increased oocyte competence in bovine. Reproduction. 2013

221)Labrecque R, Vigneault C, Blondin P,SIRARD MA. Gene expression analysis of bovine oocytes with high developmental competence obtained from FSH-stimulated animals. Mol Reprod Dev.2013

220)Cagnone GL,SIRARD MA. Transcriptomic signature to oxidative stress exposure at the time of embryonic genome activation in bovine blastocysts. Mol Reprod Dev2013

219)Sylvestre EL, Robert C, Pennetier S, Labrecque R, Gilbert I, Dufort I,  Léveillé MC,SIRARD MA. Evolutionary conservation of the oocyte transcriptome among vertebrates and its implications for understanding human reproductive function. Mol Hum Reprod.2013Jan 21.

218)Tsoi S, Zhou C, Grant JR, Pasternak JA, Dobrinsky J, Rigault P, Nieminen J, SIRARD MA, Robert C, Foxcroft GR, Dyck MK. Development of a porcine (Sus scofa) embryo-specific microarray: array annotation and validation. BMC Genomics. Aug 3;13:370.2012

217): Tesfaye D, Schellander K,SIRARD MA. Embryo genomics: Embryo Genomics meeting, Bonn, September 2011. Anim Reprod Sci. 134(1-2):1.2012

216)Gad A, Hoelker M, Besenfelder U, Havlicek V, Cinar U, Rings F, Held E, Dufort I,SIRARD MA, Schellander K, Tesfaye D. Molecular Mechanisms and Pathways Involved in Bovine Embryonic Genome Activation and Their Regulation by Alternative In Vivo and In Vitro Culture Conditions. Biol Reprod.2012

215) SIRARD MA. Factors affecting oocyte and embryo transcriptomes. Reprod Domest Anim.  Aug;47 Suppl 4:148-55.2012

214)Tsoi S, Zhou C, Grant JR, Pasternak AJ, Dobrinsky JR, Rigault P, Nieminen J,SIRARD MA, Robert C, Foxcroft GR, Dyck MK. Development of a porcine (Sus scofa) embryo-specific microarray: array annotation and validation. BMC Genomics.  3;13(1):370.2012

213)Chu T, Dufort I,SIRARD MA. Effect of ovarian stimulation on oocyte gene expression in cattle. Theriogenology.;77(9):1928-38.2012

212)Gilbert I, Robert C, Vigneault C, Blondin P,SIRARD MA. Impact of the LH surge on granulosa cell transcript levels as markers of oocyte developmental competence in cattle.Reproduction. 2012 Jun;143(6):735-47. Epub2012Mar 28.

211)Santiquet N, Vallières L, Pothier F,SIRARD MA, Robert C, Richard F.  Transplanted bone marrow cells do not provide new oocytes but rescue fertility in female mice following treatment with chemotherapeutic agents. Cell Reprogram. 14(2):123-9.2012

210)Mondou E, Dufort I, Gohin M, Fournier E,SIRARD MA. Analysis of microRNAs and  their precursors in bovine early embryonic development. Mol Hum Reprod. 2012 Apr

209)Nivet AL, Bunel A, Labrecque R, Belanger J, Vigneault C, Blondin P,SIRARD MA. FSH withdrawal improves developmental competence of oocytes in the bovine model. Reproduction. 143(2):165-71.2012

208)Cagnone GL, Dufort I, Vigneault C,SIRARD MA. Differential Gene ExpressionProfile in Bovine Blastocysts Resulting from Hyperglycemia Exposure During Early Cleavage Stages. Biol Reprod.29;86(2):Nov 9.2011[Epub ahead of print]

207)Robert C, Nieminen J, Dufort I, Gagné D, Grant JR, Cagnone G, Plourde D, Nivet AL, Fournier E, Paquet E, Blazejczyk M, Rigault P, Juge N,SIRARD MA. Combining resources to obtain a comprehensive survey of the bovine embryo transcriptome through deep sequencing and microarrays. Mol Reprod Dev.;78(9):651-64.2011

206)Labrecque R,SIRARD MA. Gene expression analysis of bovine blastocysts produced by parthenogenic activation or fertilisation. Reprod Fertil Dev.23(4):591-602.2011

205)Rekik W, Dufort I,SIRARD MA. Analysis of the gene expression pattern of bovine blastocysts at three stages of development. Mol Reprod Dev. 78(4):226-40.2011

204)Kepkova KV, Vodicka P, Toralova T, Lopatarova M, Cech S, Dolezel R, Havlicek V, Besenfelder U, Kuzmany A,SIRARD MA, Laurincik J, Kanka J. Transcriptomic analysis of in vivo and in vitro produced bovine embryos revealed a developmental change in cullin 1 expression during maternal-to-embryonic transition.  Theriogenology. 75(9):1582-95.2011

203)SIRARD MA. Follicle environment and quality of in vitro matured oocytes. J Assist Reprod Genet. . [Epub ahead of print]2011

202)Scantland S, Grenon JP, Desrochers MH,SIRARD MA, Khandjian EW, Robert C.  Method to isolate polyribosomal mRNA from scarce samples such as mammalian oocytes and early embryos. BMC Dev Biol. 15;11(1):8.2011

201)SIRARD MA.Is aneuploidy a defense mechanism to prevent maternity later in awoman's life. J Assist Reprod Genet. 28(3):209-10.2011

200)Gilbert I, Robert C, Dieleman S, Blondin P, SIRARD MATranscriptional effect of the luteinizing hormone surge in bovine granulosa cells during the peri-ovulation period.Reproduction.;141(2):193-205.2011

199)Assidi M, Montag M, Van der Ven K,SIRARD MA. Biomarkers of human oocyte developmental competence expressed in cumulus cells before ICSI: a preliminary study. J Assist Reprod Genet.;28(2):173-88.2011

198)Assidi M,Dieleman S,SIRARD MACumulus cells gene expression following the LH surge in bovine preovulatory follicles: potential early markers of oocyte competence. Reproduction,;140(6):835-52.2010

197)Sylvestre E , Pennetier S, Bureau M, Robert C ,SIRARD MAInvestigating the Potential of Genes Preferentially Expressed in Oocyte to Induce Chromatin Remodeling in Somatic Cells. Cellular Reprogramming: 12 (5) 1-12.2010

196)Seli E, Robert C,SIRARD MA. OMICS in assisted reproduction: possibilities and pitfalls. Mol Hum Reprod. Aug;16(8):513-30.2010

195)Hamel M, Dufort I, Robert C, Léveillé MC, Leader A,SIRARD MAIdentification of follicular marker genes as pregnancy predictors for human IVF: new evidence for the involvement of luteinisation process. Mol Hum Reprod.Aug;16(8):548-56.2010

194)Gilbert I, Scantland S, Sylvestre EL, Dufort I,SIRARD MA, Robert C. Providing a stable methodological basis for comparing transcript abundance of developing embryos using microarrays. Mol Hum Reprod. Aug;16(8):601-16. 2010

193)Jiang JY, Xiong H, Cao M, Xia X,SIRARD MA, Tsang BK. Mural granulosa cell gene expression associated with oocyte developmental competence. J Ovarian Res.2010Mar 6;3:6

192)Hamel M, Dufort I, Robert C, Leveillé MC, Leader A, Sirard MA. Genomic assessment of follicular marker as pregnancy predictors for human IVF. Mol Hum Reprod.Feb;16:87-96.2010

191) Vallée M, Dufort I, Desrosiers S, Labbe A, Gravel C, Gilbert I, Robert C, SIRARD MA. Revealing the bovine embryo transcript profiles during early in vivo embryonic development. Reproduction.2009Jul;138(1):95-105. Epub 2009 Apr 21.190) Gilbert I, Scantland S, Sylvestre EL, Gravel C, Laflamme I,SIRARD MA, Robert  C. The dynamics of gene products fluctuation during bovine pre-hatching development. Mol Reprod Dev.2009 Aug;76(8):762-72.189) Gilbert I, Scantland S, Dufort I, Gordynska O, Labbe A,SIRARD MA, Robert C.  Real-time monitoring of aRNA production during T7 amplification to prevent the loss of sample representation during microarray hybridization sample preparation. Nucleic Acids Res.2009May;37(8):e65. Epub 2009 Mar 30.188) Tesfaye D, Ghanem N, Carter F, Fair T,SIRARD MA, Hoelker M, Schellander K,  Lonergan P. Gene expression profile of cumulus cells derived from cumulus-oocyte  complexes matured either in vivo or in vitro. Reprod Fertil Dev.  2009;21(3):451-61.

187)Christian Vigneault, Catherine Gravel, Maud Vallée, Serge McGraw andSIRARD MAUnveiling the bovine embryo transcriptome during the maternal to embryonic transition. Reproduction.2009Feb;137(2):245-57

186)Vigneault C, McGraw S, Sirard MA. Spatio-temporal expression of transcriptional regulators in concert with the maternal to embryonic transition during bovine in vitro embryogenesis.  Reproduction. 2009Jan;137(1):13-21.

185) Paré J, Audet-Grenier MH, Rouillier P,SIRARD MA. Evaluation of the DG29 test  for early detection of pregnancy in cattle. Can Vet J.2008Nov;49(11):1119-21

184)Julie Paré, Marie-Hélène Audet-Grenier, Paul Rouillier and  SIRARD MA. Evaluation of the DG29 test for early detection of pregnancy in cattle. Can Vet J;49:1119–1121.2008

183) Campagna C, Ayotte P,SIRARD MA, Bailey JL. An environmentally relevant mixture of organochlorines, their metabolites and effects on preimplantation development of porcine embryos.  Reprod Toxicol;25(3):361-6.2008182) Assidi M, Dufort I, Ali A, Hamel M, Algriany O, Dielemann S,SIRARD MA. Identification of Potential Markers of Oocyte Competence Expressed in BovineCumulus Cells Matured with Follicle-Stimulating Hormone and/or PMA In Vitro. Biol Reprod. 79(2):209-22.2008181) Vallée M, Aiba K, Piao Y, Palin MF, Ko MS,SIRARD MA. Comparative analysis of oocyte transcript profiles reveals a high degree of conservation among species.  Reproduction. Apr;135(4):439-48.2008180) Bonnet A, Dalbiès-Tran R,SIRARD MA. Opportunities and challenges in applying genomics to the study of oogenesis andfolliculogenesis in farm animals. Reproduction.135:119-28. 2008179) Torner H, Ghanem N, Ambros C, Hölker M, Tomek W, Phatsara C, Alm H,SIRARD MA, Kanitz W, Schellander K, Tesfaye D. Molecular and subcellular characterisation of oocytes screened for their developmental competence based on glucose-6-phosphate dehydrogenase activity. Reproduction. 135(2):197-212.2008178) Hamel M, Dufort I, Robert C, Gravel C, Leveille MC, Leader A,SIRARD MA. Identification of differentially expressed markers in human follicular cells associated with competent oocytes. Hum Reprod;23(5):1118-27.2008177) McGraw S, Morin G, Vigneault C, Leclerc P,SIRARD MA. Investigation of MYST4 histone acetyltransferase and its involvement in mammalian gametogenesis. BMC Dev Biol.2;7:123.2008176) Reyes-Moreno C, Laflamme J, Frenette G,SIRARD MA, Sullivan R. Spermatozoa modulate epididymal cell proliferation and protein secretion in vitro. Mol Reprod Dev.75(3):512-20. 2008175) Ghanem N, Holker M, Rings F, Jennen D, Tholen E,SIRARD MA, Torner H, Kanitz W, Schellander K, Tesfaye D. Alterations in transcript abundance of bovine oocytes recovered at growth and dominance phases of the first follicular wave. BMC Dev Biol.2007 Jul 27;7:90.174) Dessie SW, Rings F, Holker M, Gilles M, Jennen D, Tholen E, Havlicek V, Besenfelder U, Sukhorukov VL, Zimmermann U, Endter JM,SIRARD MA, Schellander K, Tesfaye D. Dielectrophoretic behavior of in vitro-derived bovine metaphase II oocytes and zygotes and its relation to in vitro embryonic developmental competence and mRNA expression pattern. Reproduction.2007 May;133(5):931-46.173)SIRARD MA Desrosier S, Assidi M. In vivo and in vitro effects of FSH on oocyte maturation and developmental competence. Theriogenology.2007 Sep 1;68 Suppl 1:S71-6. Epub 2007 Jun 22.172)SIRARD MA. From biological fundamentals to practice, and back. Theriogenology. 2007 Sep 1;68 Suppl 1:S250-6. Epub2007 Jun 15.171)  McGraw S, Vigneault C,SIRARD MA. Temporal expression of factors involved in chromatin remodeling and in gene regulation during early bovine in vitro embryo development.  Reproduction. 2007 Mar;133(3):597-608.170)  Campagna C, Ayotte P,SIRARD MA, Arsenault G, Laforest JP, Bailey JL. Effect of an environmentally relevant metabolized organochlorine mixture on porcine cumulus-oocyte complexes.  Reprod Toxicol.2007Feb;23(2):145-52. Epub 2006 Dec 8.169) Vigneault C, Gilbert I,SIRARD MA, Robert C.  Using the histone H2a transcript as an endogenous standard to study relative transcript abundance during bovine early development.  Mol Reprod Dev.2007Jun;74(6):703-15.

Other contributions (Book chapters)

 

Dufort I, Robert C andSIRARD MA"Studying bovine early embryo transcriptome by microarray", Nuclear Reprogramming in "Methods in Molecular Biology", 1-16, Humana Press Editors: Alice JOUNEAU, Hélène JAMMES, Dr Nathalie BEAUJEAN2014

SIRARD MA, Gilbert I. Omics for oocyte selection Oocyte pathology " Biology and Pathology of the Oocyte."   Edited by alan Trounson. Cambridge University Press 2013297-305

SIRARD MA  Screening for oocyte competence. In“Oocyte Physiology and Development in Domestic Animals”. R Krisher ed. Wiley-Blackwell,John Wiley & Sons. 2011 184-198.2013

Assidi M andSIRARD MA . Cumulus cells' gene expression as a manifestation of oocyte quality.In: Mammalian oogenesis. Eds: G. Coticchio, L. De Santis, D. Albertini. Elsevier.2012 

SIRARD MA, Mammalian embryonic genome activation in Reproduction in Domestic Ruminants VII.  Smith, Lucy, Pate and Spencer (Eds). Nottingham University Press. 2011. 212-225.

SIRARD MA, Genomic Regulation through RNA in Oocyte Maturation of Large Mammals. Oocyte Maturation and Fertilization: A Long History for a Short Event,2010, 71-79



Mes prix et distinctions

  • Prix Synergie du Canada 2000

Mes recherches en images